攝食抑制因子1(NES1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Nesfatin 1 (NES1)
- 編號(hào)UCEA242Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時(shí)長2h
- 檢測范圍493.8-40,000pg/mL
- 靈敏度最小可檢測劑量小于等于183.4pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1361 ¥ 1944 ¥ 8748 ¥ 16524 ¥ 136080
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測攝食抑制因子1(NES1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的攝食抑制因子1(NES1)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-101 | 86 |
| EDTA plasma(n=5) | 96-104 | 101 |
| heparin plasma(n=5) | 87-96 | 90 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的攝食抑制因子1(NES1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中攝食抑制因子1(NES1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-98% | 80-90% | 96-103% | 83-101% |
| EDTA plasma(n=5) | 83-90% | 96-103% | 97-105% | 84-103% |
| heparin plasma(n=5) | 78-94% | 82-89% | 80-101% | 87-96% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將攝食抑制因子1(NES1)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計(jì)算樣品濃度。
贈(zèng)品
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA242Mu01 | 攝食抑制因子1(NES1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA242Mu01 | 攝食抑制因子1(NES1)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA242Mu01 | 攝食抑制因子1(NES1)多克隆抗體 | WB; IHC |
| USEA242Mu | 攝食抑制因子1(NES1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA242Mu | 攝食抑制因子1(NES1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA242Mu | 攝食抑制因子1(NES1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA242Mu | 攝食抑制因子1(NES1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Bulletin of Clinical Psychopharmacology | Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic Disorder [Psikofarmakoloji: 2043] |
| Progress in Neuro-Psychopharmacology & Biological Psychiatry | High plasma nesfatin-1 level in patients with major depressive disorder [PubMed: 21163317] |
| Endocrine | Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus [SpringerLink: h978088657555w16] |
| Bulletin of Clinical Psychopharmacology | Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder [Psikofarmakoloji: 2212] |
| Psychoneuroendocrinology. | Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder [PubMed: 22595767] |
| Iranian Journal of Health and Physical activity | Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats [Ijvst: Source] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Decreased serum nesfatin-1 levels in endometriosis [Pubmed: 24702902] |
| Bulletin of Clinical Psychopharmacology | Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder. [Psikofarmakoloji: Source] |
| Current Pharmaceutical Design | Role of NUCB2/nesfatin-1 as a possible biomarker. [Pubmed: 23537082] |
| Regulatory, Integrative and Comparative Physiology | A role of nesfatin-1/NucB2 in dehydration-induced anorexia [Pubmed:24829503] |
| J Endocrinol Invest. | Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndrome [Pubmed:24920281] |
| Int J Surg. | The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study. [Pubmed:25448651] |
| Hormone molecular biology and clinical investigation | The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers [Pubmed:25581765] |
| Turk J Gastroenterol. | New hormones to predict the severity of gallstone-induced acute pancreatitis [Pubmed:25599787] |
| Respiration | Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1? [PubMed: 26088934] |
| J Clin Res Pediatr Endocninol | Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children [PubMed: 26831553] |
| Bosn J Basic Med Sci | Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndrome [PubMed: 26295295] |
| Original Article / Orijinal Makale | Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1 [Sayilar: 294] |
| Gynecol Endocrinol | Nesfatin-1 levels and metabolic markers in polycystic ovary syndrome [PubMed: 26062107] |
| International Journal of Neuroscience | Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage* [Pubmed:26872406] |
| 未找到 | Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairment [P.1.e.012.pdf] |
| International Journal of Developmental | Serum?nesfatin-1?levels: a?potential?new?biomarker?in?patients?with?subarachnoid?hemorrhage. [pubmed:26872406] |
| Current Pharmaceutical Design | Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Disease [pubmed:28137218] |
| Arch Endocrinol Metab | Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes [pubmed:28977160] |
| Indian Journal of Psychiatry | Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving? [pubmed:28529367] |
| Biomedicine & Pharmacotherapy | Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ [Pubmed:29604590] |
| Biochimica et?Biophysica?Acta | Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodeling [Pubmed:29627363] |
| BMC Psychiatry | Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patients [Pubmed:29615007] |
| Probiotics?and Antimicrobial?Proteins | Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar [Pubmed: 30232744] |
| An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages [] | |
| clinical neurology and neurosurgery | Relationsh?p of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels [Pubmed: 32311618] |
| Ann Nutr Metab | Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity [Pubmed: 33027789] |
| Endokrynologia Polska | Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke [33283259] |
| Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder [] |
