波形蛋白(VIM)單克隆抗體
Monoclonal Antibody to Vimentin (VIM)
- 編號UMAB040Hu22
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C12
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPB040Hu01-波形蛋白(VIM)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠), Oryctolagus cuniculus (Rabbit，兔), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬)
- 應用WB; IHC; ICC/IF
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 315 ¥ 735 ¥ 1050 ¥ 2625 ¥ 10500
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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FITC staining on IF; Samples: Human MCF7 Cells; Primary Ab: 40μg/ml Mouse Anti-Human VIM Antibody Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
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DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Samples: Human Endometrial cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Vector Red staining on IHC-P;
Samples: Human Testis Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Monoclonal Antibody -
FITC and CY3 staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 40μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18) -
Cy3 staining on IF;
Sample: Primary Canine Bladder Stromal Fibroblasts cell
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/ml Cy3-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu16) -
Cy3 staining on IF;
Sample: Primary Rabbit Neonatal Dermal Fibroblasts cell
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/ml Cy3-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu16) -
Cy3 staining on IF;
Sample: Primary Rabbit Corneal Stromal cells cell
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/ml Cy3-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu16) -
FITC staining on IF;
Sample: Primary Rabbit Myocardial Cells cell
Primary Ab: 10μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
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Figure. Western Blot; Sample: Recombinant VIM, Human.
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Western Blot; Sample: Rat Heart lysate;
Primary Ab: 2μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 2μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Porcine Heart lysate;
Primary Ab: 2μg/ml Mouse Anti-Human VIM Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對VIM的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別VIM。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-40μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPB040Hu02 | 波形蛋白(VIM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB040Hu01 | 波形蛋白(VIM)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPB040Hu01 | 波形蛋白(VIM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPB040Hu61 | 波形蛋白(VIM)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPB040Hu62 | 波形蛋白(VIM)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB040Hu02 | 波形蛋白(VIM)多克隆抗體 | IHC; ICC/IF |
| UPAB040Hu01 | 波形蛋白(VIM)多克隆抗體 | WB; IHC; ICC/IF |
| ULAB040Hu71 | 波形蛋白(VIM)多克隆抗體(生物素標記) | WB |
| UMAB040Hu24 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB040Hu25 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB040Hu21 | 波形蛋白(VIM)單克隆抗體 | WB; IHC |
| UMAB040Hu23 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB040Hu27 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB040Hu22 | 波形蛋白(VIM)單克隆抗體 | WB; IHC; ICC/IF |
| UFAB040Hu02 | 抗波形蛋白(VIM)單克隆抗體 | FCM |
| UFAB040Hu06 | 抗波形蛋白(VIM)單克隆抗體 | FCM |
| UFAB040Hu04 | 抗波形蛋白(VIM)單克隆抗體 | FCM |
| UFAB040Hu08 | 抗波形蛋白(VIM)單克隆抗體 | FCM |
| USEB040Hu | 波形蛋白(VIM)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB040Hu | 波形蛋白(VIM)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB040Hu01 | 波形蛋白(VIM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Cancer?Epidemiology,?Biomarkers?& Prevention | Aberrant Vimentin Methylation Is Characteristic of Breast Cancer [pubmed:22315367] |
| Scientific Reports | Glyoxalase?1-knockdown?in?human?aortic?endothelial?cells - effect on the?proteome?andendothelial?function?estimates. [pubmed:27898103] |
| Journal of?Proteomics | HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin [Pubmed:29477555] |
| Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy [Pubmed: 31511328] |
| American Journal of Transplantation | Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allografts [Pubmed: 31729155] |
| Lung | Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development? [Pubmed: 32088750] |
| Cell Biol Toxicol | ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer [33566221] |
| CELL BIOLOGY AND TOXICOLOGY | Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence [33758996] |
| Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women [] | |
| Advanced glycation end-products associate with podocytopathy in type II diabetic patients [] | |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… [] |
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