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促生長(zhǎng)激素釋放激素(GHRH)活性蛋白

Active Growth Hormone Releasing Hormone (GHRH)

GRF; GHRF; Somatocrinin; Growth-Hormone-Releasing Factor; Sermorelin; Somatoliberin

  • 促生長(zhǎng)激素釋放激素(GHRH)活性蛋白 產(chǎn)品包裝(模擬)
  • 促生長(zhǎng)激素釋放激素(GHRH)活性蛋白 產(chǎn)品包裝(模擬)
  • APA438Hu02.jpg Figure. SDS-PAGE
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Growth Hormone Releasing Hormone (GHRH), also known as somatocrinin, is a peptide hormone secreted by the hypothalamus. It binds to specific receptors in the pituitary gland, stimulating the synthesis and release of growth hormone (GH), which regulates growth, metabolism, and cellular functions.Growth Hormone Releasing Hormone Receptor (GHRHR) is a transmembrane receptor located on the surface of pituitary cells. It has a specific binding site that can precisely recognize and bind to GHRH.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GHRH and recombinant human GHRHR.Briefly, biotin-linked GHRH were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to GHRHR-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of GHRH and GHRHR was shown in Figure 1, the EC50?for this effect is 0.228ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
URPA438Hu02 促生長(zhǎng)激素釋放激素(GHRH)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPA438Hu01 促生長(zhǎng)激素釋放激素(GHRH)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UAPA438Hu02 促生長(zhǎng)激素釋放激素(GHRH)活性蛋白 Cell?culture;?Activity?Assays.
UPAA438Hu01 促生長(zhǎng)激素釋放激素(GHRH)多克隆抗體 WB
ULAA438Hu71 促生長(zhǎng)激素釋放激素(GHRH)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
UMAA438Hu21 促生長(zhǎng)激素釋放激素(GHRH)單克隆抗體 IHC
UMAA438Hu22 促生長(zhǎng)激素釋放激素(GHRH)單克隆抗體 IHC
UCEA438Hu 促生長(zhǎng)激素釋放激素(GHRH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
ULMA438Hu 促生長(zhǎng)激素釋放激素(GHRH)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UKSA438Hu11 促生長(zhǎng)激素釋放激素(GHRH)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
19 Role of GHRH in Sleep and Growth Impairments Induced by Upper Airway Obstruction in Rats [Ers: 00197610]
19 Role of growth hormone-releasing hormone in sleep and growth impairments induced by upper airway obstruction in rats [PubMed: 21406516]
Global Veterinaria Effect of Moringa Oleifera Extract on Nicotine Induced Neurotoxicity in Female Rat and Their Embryos [Idosi: Source]
Cell?Reports Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice [Pubmed: 30404008]
Drug?Testing?and?Analysis An Immuno‐PCR screen for the detection of CJC‐1295 and other Growth Hormone Releasing Hormone analogues in equine plasma [Pubmed: 30489688]
Sleep and Breathing Effects of somatotropic axis on cognitive dysfunction of obstructive sleep apnea [Pubmed: 31073904]
Diabetes Lack of brain insulin receptor substrate-1 causes growth retardation, with decreased expression of growth hormone-releasing hormone in the hypothalamus. [33980693]
Sci Rep Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patients [Pubmed:35508633]
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