甘氨酸脫氫酶(GLDC)活性蛋白
Active Glycine Dehydrogenase (GLDC)
GCSP; NKH; Decarboxylating; Glycine Decarboxylase; Glycine Cleavage System Protein P; Glycine dehydrogenase (aminomethyl-transferring)
- 編號UAPG475Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點6.6
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Glycine Dehydrogenase (GLDC) is a key mitochondrial enzyme involved in the catabolism of glycine. It catalyzes the oxidative decarboxylation of glycine, playing an important role in one-carbon metabolism. GLDC provides one-carbon units for various cellular processes like nucleotide synthesis and methylation reactions.Besides,Apolipoprotein H (APOH) has been identified as an interactor of GLDC,Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse GLDC and recombinant mouse AGXT. Briefly, GLDC was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to AGXT-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GLDC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse GLDC and recombinant mouse AGXT was shown in Figure 1, the EC50?for this effect is 0.097ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPG475Mu01 | 甘氨酸脫氫酶(GLDC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPG475Mu02 | 甘氨酸脫氫酶(GLDC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPG475Mu01 | 甘氨酸脫氫酶(GLDC)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAG475Mu01 | 甘氨酸脫氫酶(GLDC)多克隆抗體 | IHC |
| UPAG475Mu02 | 甘氨酸脫氫酶(GLDC)多克隆抗體 | WB; IHC; ICC; IP. |
| USEG475Mu | 甘氨酸脫氫酶(GLDC)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMG475Mu | 甘氨酸脫氫酶(GLDC)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Chemico-Biological Interactions | Long-term co-exposure DBP and BaP causes imbalance in liver macrophages polarization via activation of Notch signaling regulated by miR-34a-5p in rats [Pubmed:35378083] |
