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血管緊張素原(AGT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Angiotensinogen (AGT)

ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)

  • 血管緊張素原(AGT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 血管緊張素原(AGT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對血管緊張素原(AGT)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
URPA797Hu02 血管緊張素原(AGT)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
URPA797Hu01 血管緊張素原(AGT)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UAPA797Hu61 血管緊張素原(AGT)活性蛋白 Cell?culture;?Activity?Assays.
UEPA797Hu61 血管緊張素原(AGT)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAA797Hu01 血管緊張素原(AGT)多克隆抗體 WB; IHC
UPAA797Hu02 血管緊張素原(AGT)多克隆抗體 WB; IHC; ICC/IF
UMAA797Hu22 血管緊張素原(AGT)單克隆抗體 WB; IHC; ICC; IP.
USEA797Hu 血管緊張素原(AGT)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
UMEA797Hu 血管緊張素原(AGT)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
USCA797Hu 血管緊張素原(AGT)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
ULMA797Hu 血管緊張素原(AGT)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
UKSA797Hu01 血管緊張素原(AGT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Transplant International Elevated urinary angiotensinogen a marker of intrarenal renin angiotensin system in hypertensive renal transplant recipients: does it play a role in development of proteinuria in hypertensive renal transplant patients? [Wiley: source]
Amyloid The relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patients [PubMed: 22320202]
Pediatric Mephrology Urinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertension [PubMed: PMC3661928]
Acta Paediatrica Urinary angiotensinogen as a novel marker of obstructive nephropathy in children [Pubmed: 23772991]
Journal of the Renin-Angiotensin-Aldosterone System Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia [Sagepub: Source]
Pediatric Nephrology MP707 CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRY [Oxfordjournals:Source]
journal of human hypertension Relationship between blood pressure var [PubMed: 26223347]
PLoS One Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes Analysis [PubMed: 26098644]
MKA Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasa [Index.Php: Art]
Journal of Human Hypertension Relationship between blood pressure variability and renal activity of the renin-angiotensin system. [pubmed:26223347]
Acta Pharmacol Sin. Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells. [pubmed:27890915]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
Life?Sciences Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity [Pubmed:30036488]
American Journal of Physiology-Renal?Physiology Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in Rats [Pubmed:29846109]
Methods in Molecular Biology Quantitation of Total and Free Thiol Angiotensinogen as a Prognostic Marker for Preeclampsia [Pubmed: 31069778]
J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555]
Hypertens Res Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents [33568792]
Physiol Rep (Pro) renin receptor antagonist PRO20 attenuates nephrectomy?\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/|??\catenin signaling [34057312]
Cancer Cell Int A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer [34215253]
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